FIREScript® is an improved version of Murine Moloney Leukemia Virus (M-MLV) reverse transcriptase (RT) with exceptional stability at room temperature, improved thermostability, substantially faster synthesis rates and higher sensitivity compared to the wild- ype M-MLV RT. FIREScript® is used for first strand cDNA synthesis from total RNA or purified mRNA and is compatible with wide range of reaction temperatures.

✓ stable at room temperature for 30 days
✓ suitable for working between 37-60°C
✓ cDNA synthesis completed in 15 minutes
✓ detecting total RNA amounts from 0.01 ng
✓ generates full length cDNA of at least 8 kb
✓ full RNase H activity
✓ available in convenient mix and flexible kit formats
✓ reaction set-up and shipment without ice

FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase.

This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C-60°C.
FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).

• FIREScript® Reverse Transcriptase (200 U/μl)
• 10x RT Reaction Buffer with DTT (500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT)

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature

• FIREScript® Reverse Transcriptase 1x40000 U | 200 µl
• 10x RT Reaction Buffer with DTT 1x500 µl

Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.