HOT FIREPol® DNA Polymerase
- Hot-start DNA Polymerase with unique 30-day room temperature stability for your everyday PCR needs.
A chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only by pre-incubation at 95°C, preventing any unspecific polymerase activity at lower temperatures during reaction set-up.
✓ increased specificity and sensitivity
✓ reduced primer-dimer formation
✓ reaction set-up and shipment without ice
OVERVIEW
Chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only after heat treatment at 95°C which prevents any unspecific polymerase activity at lower temperatures during reaction set-up. HOT FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl₂ and an additive for difficult templates. HOT FIREPol® 10x Buffer B2 contains non-ionic detergent suppressing inhibitory effects of the trace of DNA extraction buffers and enhancing PCR yield and efficiency. |
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DESCRIPTION |
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HOT FIREPol® is a chemically modified FIREPol® DNA Polymerase. |
KITS COMPONENT |
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• HOT FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers. |
STORAGE & SHIPPING |
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Storage: Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product |
REAGENT PROVIDED |
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• HOT FIREPol® DNA Polymerase 1x500 U | 100 µl |
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CITATION
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