- Unbiased Lysis: Efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungi, protozoans, and viruses from any sample including feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.
- Ultra-Pure: High-quality DNA/RNA (including mall/microRNAs) are inhibitor-free and ready for RT/qPCR and microbiome measurements using Next-Gen sequencing.
- High Sensitivity: Increased detection limit of very low abundance organisms.
The ZymoBIOMICS MagBead DNA/RNA kit provides a high-throughput, magnetic bead-based purification of both high-quality DNA and total RNA (including small/microRNAs) from the same starting sample. The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. The extraction method utilizes magnetic beads for DNA/RNA extraction without the use of phenol and is eluted into =50 µl of ZymoBIOMICS DNase/RNase-Free Water. DNA/RNA is ready for any downstream application including Next-Gen Sequencing, RT-qPCR,, etc.
|Binding Capacity||15 µg DNA/RNA per 30 µl ZymoBIOMICS MagBinding Beads.|
|Equipment Needed||Magnetic stand or separator, heat block, liquid handler or robotic sample processer (user provided).|
|Purity||High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc.|
|Recommended Materials||(sold separately) – 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005).|
|Sample Preservation||DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.|
|Sample Types||Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from = 50 mg of mammalian feces, = 50 mg soil, and 5-20 mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs.|
|Size Limits||Capable of recovering DNA and total RNA ≥ 17 nucleotides.|
|Storage||DNA and RNA eluted with ZymoBIOMICS DNase/RNase-Free Water (provided)can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage.|
ZymoBIOMICS MagBead DNA/RNA