The Zymo-Seq UDI Primer Sets contain pre-mixed Unique Dual Index (UDI) primers that can be used to barcode any library containing TruSeq® adapters. Indexes in the Zymo-Seq UDI Primer Set (Indexes 1-12) are dispensed in 1.5 mL tubes (Cat. No. D3008), and the Zymo-Seq UDI Primer Plate (Indexes 1-96) are dispensed in single-use foil-sealed 96-well plates (Ca. No. D3096). Indexed libraries are compatible with any Illumina® sequencer. These indexes are compatible with the Zymo-Seq RiboFree Total RNA Library Kit.


Each tube contains a pre-mixed forward and reverse primer set that contain a unique i5 and i7 index, respectively. The concentration of each UDI primer pre-mix is 5 µM total (2.5 µM each primer).
Please view the “Zymo-Seq UDI Primer Set 1-96” spreadsheet for index sequences and adapter trimming parameters.
No, these index primers only work with TruSeq compatible libraries.

Zymo-Seq UDI Primer Sets

Cat # Name Size
D3008 Zymo-Seq UDI Primer Set (Indexes 1-12) 12 indexes
D3096 Zymo-Seq UDI Primer Plate (Indexes 1-96) 96 Indexes