Highlights
- Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
- DNA-Free: DNase I included.
- NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing,
RT-qPCR, hybridization, etc.
Description
The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin™ Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.
Technical Specifications
| Equipment | Microcentrifuge, vortex |
|---|---|
| Purity | RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
| Sample Source | Cells or tissue samples, yeast, plant or bacteria. Compatible with DNA/RNA Shield™ and RNAlater™. |
| Size Range | Total RNA ≥ 17 nt |
| Yield | Microprep: 10 µg RNA (binding capacity), ≥6 µl (elution volume) |
Product FAQ
Q1: Is DNase I available for individual purchase?
Yes, the catalog number for the DNase I set (DNase and DNA Digestion Buffer) that we offer is E1010.
Q2: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q3: Is it possible to extract proteins with the Quick-RNA kit?
Yes, proteins can be acetone precipitated from the column flowthrough. Please request supplementary protocol from Zymo Research Technical Support.
Q4: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q5: Will the kit isolate small RNAs?
Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.
Q6: Can samples be stored in RNA Lysis Buffer prior to processing?
Yes, samples in RNA Lysis Buffer are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q7: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q8: What is the difference between the Quick-RNA Miniprep and the Quick-RNA Miniprep Plus?
Use the Quick-RNA Miniprep for cells and soft tissues.
The “Plus” kit accommodates all sample types (cells, tissue, blood) and comes with DNA/RNA Shield (sample collection, transport, storage at ambient temperature).
Q9: I ran out of RNA Wash Buffer. Can I use something else?
Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
Citations
Reviews
“RNA isolation from tissue culture cells used to be my most hated protocol – labor intensive, tedious, and the horrible smell of the BME used in the protocol. This kit is much faster and easier and no horrible smells! Both the RNA yield and the real time results are just as good as our previous kit.”
– Lisa G. (University of Virginia)
“Amazing results, the only RNA extraction kit I ever buy now. In my opinion, there is no product on the market that is as good a value and as effective as this kit for plant tissue RNA extractions.”
– Erin B. (Occidental College)
“Good price and easy to use, plus good quality. It is compatible to QIAGEN products RNeasy kit, but with reasonable price and equal quality. Especially the DNase is inexpensive and is included in the kit. No matter animal RNA, or plant RNA, or bacterial RNA, all could use with this one kit.”
– Xiaolu J. (Florida Atlantic University)
“We are able to quickly and easy extract high quality RNA for use in RT-PCR. This kit is very affordable as well!.”
– Bo B. (Drew University)
“Easy and simple to use, even students can manage this! Reliable and in terms of value it is excellent. Color coded spin columns make it fool proof.”
– Jody H. (Otago University)
Kit Components
| Cat # | Name | Size |
|---|---|---|
|
W1001-10 |
DNase/RNase-Free Water | 10 ml |
|
W1001-4 |
DNase/RNase-Free Water | 4 ml |
|
R1003-3-24 |
RNA Wash Buffer | 24 ml |
|
R1003-3-48 |
RNA Wash Buffer | 48 ml |
|
R1060-1-50 |
RNA Lysis Buffer | 50 ml |
|
R1060-2-100 |
RNA Prep Buffer | 100 ml |
|
R1060-2-25 |
RNA Prep Buffer | 25 ml |
|
C1001-50 |
Collection Tubes | 50 Pack |
|
C1004-50 |
Zymo-Spin IC Columns | 50 Pack |
|
E1010-1-4 |
DNA Digestion Buffer | 4 mL |
|
E1010-1-16 |
DNA Digestion Buffer | 16 mL |
