Description
The Quick-RNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of RNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the RNA that is ideal for downstream molecular-based applications including Next-Gen sequencing, RT-PCR, microarray, hybridization, etc.
Technical Specifications
| Applicable For |
For all sensitive downstream applications, such as Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc. |
| Elution Volume |
25 µl |
| Equipment |
Microcentrifuge, vortex, cell disrupter/pulverizer |
| Processing Time |
≤ 10 minutes |
| Processing Volume |
50-100 mg (wet weight) fungi or bacteria. 109 bacterial cells and 108 yeast cells |
| Purity |
RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc.
A260/A280, A260/A230: > 1.8. |
| Sample Source |
Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled. |
| Size Range |
Total RNA ≥ 17 nt |
| Type |
Total RNA |
| Yield |
Miniprep
50 µg RNA (binding capacity)
≥ 25 µl (elution volume) |
