- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased: Complete RNA recovery without miRNA loss.
Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA.
The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.).
The entire procedure typically takes only 7 minutes.
|Compatibility||TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.|
|Sample Inactivation||TRI Reagent® (provided with R2061, R2063) inhibits RNase activity and inactivates viruses and other infectious agents.|
|Sample Source||Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).|
|Size Range||Total RNA ≥ 17 nt|
|Yield||10 µg RNA (binding capacity), ≥6 µl (elution volume)|
Q1: Is Direct-zol suitable for very small numbers of cells?
Q2: Is DNase I available for individual purchase?
Q3: How to store DNase-I following resuspension?
Q4: Is the DNase-I treatment necessary?
Q5: Is the kit compatible with samples stored in DNA/RNA Shield?
Q6: Is it possible to extract proteins with the Direct-zol RNA kits?
Q7: I ran out of RNA Wash Buffer. Can I use something else?
Q8: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Q9: Is it possible to isolate DNA with the Direct-zol RNA kits?
Q10: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Q11: Which phenol-based reagents are compatible with Direct-zol?
Q12: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Q13: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
“Before I discovered this kit, I was isolating RNA the old school way with chloroform and it would take half the day to finish the protocol. The Direct-zol RNA Miniprep kit is AWESOME! It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench.”
– A. Newhart (The Wistar Institute)
“Simple protocol and yielded good quality of RNA. Only one kit working for all type of tissue, cell and especially biological fluids.”
– Mohan K. (University of Illinois, Chicago)
“Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? Just one column and two buffers, I love it.”
– Arjan V. (Indiana University)
Direct-zol RNA Microprep Kits