The Zymoclean Gel RNA Recovery Kit provides a quick and efficient purification method for recovery of RNA fragments from agarose gels. The procedure combines a unique, single-step agarose dissolving/RNA binding buffer with Zymo-Spin column technology to yield high quality, purified RNA in just minutes. The purified RNA is eluted into small volumes of DNase/RNase-free water for highly concentrated samples suitable for subsequent RNA-based manipulations. Compatible with MOPS, TAE, and TBE buffered agarose gels (formaldehyde up to 2.0%).

Equipment	Microcentrifuge, 37°C-65°C heat source
Purity	RNA is ready for all subsequent analysis and molecular manipulation
Sample Source	Single- or double-stranded RNA fragments (≥200 nt) resolved in TAE TBE, and MOPS buffered agarose gels. Compatible with formaldehyde to 2.0% (final conc.)
Size Range	RNA fragments ≥500 nt (≥80% recovery)
Yield	10 µg RNA (binding capacity), ≥6 µl (elution volume)

Q1: Are the columns and wash buffer interchangeable with the RCC-5 Kit?

Q2: Can the Zymoclean be used for samples in solution (without running on a gel)?

Q3: What is the maximum weight of gel slice I can use?

Q4: I ran out of RNA Wash Buffer. Can I use something else?

Q5: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?