- Efficient: Recovery of ss- or ds- RNA (and DNA) fragments (17-200 nt) from up to 20% (w/v) polyacrylamide gels.
- Concentrated: Up to 10 µg sample in = 6 µl elution.
- Compatible with up to 25% (w/v) polyacrylamide.
The ZR small-RNA PAGE Recovery Kit provides an easy and efficient method for the extraction of high quality small RNAs from polyacrylamide gels (native and/or denatured). The ZR small-RNA™ PAGE Recovery Kit is a refinement of the “crush and soak” method that incorporates a unique buffer system together with Zymo-Spin column technologies for improved recovery and added convenience. The recovered RNA can be concentrated into volumes as small as 6 µl, making it ideal for many downstream enzymatic reactions and manipulations.
|Equipment||Microcentrifuge, 37°C-65°C heat source, dry ice or -80°C freezer|
|Purity||RNA is ready for all subsequent analysis and molecular manipulation|
|Sample Source||RNA or DNA fragments (17-200 nt) resolved in PAGE gels (up to 25%), also compatible with TBE-, denatured, urea PAGE gels. Compatible with ethidium bromide or ssRNA-specific dyes (e.g. GelStar).|
|Size Range||RNAs 17-200 nt (fragments 17-28 nt is ≥50 % recovery)|
|Yield||10 µg RNA (binding capacity), ≥6 µl (elution volume)|
Q1: What is the recovery rate?
Q2: Can the kit be used to recover DNA fragments run on a PAGE gel?
Q3: Can the kit be used to purify DNA/RNA fragments > 200 nucleotides run on a PAGE gel?
Q4: Is the kit compatible with TBE-Urea PAGE?
Q5: What is the maximum amount of gel that can be processed per prep?
Q6: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?
ZR small-RNA PAGE Recovery Kit