Description
The Quick-RNA Tissue/Insect Microprep Kit provides for rapid isolation of total RNA from various insect and arthropod specimens (e.g. mosquitoes, bees, lice, ticks, Drosophila melanogaster). The Quick-RNA Tissue/Insect Microprep Kit employs ultra-high density ZR BashingBeads for sample homogenization and a robust buffer system delivering total RNA (including small RNAs) as well as DNA removal from a variety of samples.
The Zymo-Spin IIIC Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin IC Column efficiently binds total RNA. The DNase/RNase-Free Water eluted RNA is suitable for subsequent procedures including RT-PCR.
Technical Specifications
Equipment |
Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional) |
Purity |
RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
Sample Source |
Small amounts (n ≥ 1 and ≤ 10 mg) of insect and arthropod specimens (e.g. mosquitoes, bees, lice, ticks, Drosophila melanogaster) |
Size Range |
Total RNA ≥ 17 nt |
Yield |
10 µg RNA (binding capacity), ≥6 µl (elution volume) |