Chloroplasts are membrane-bound organelles found in plant and algal cells which perform the function of photosynthesis. Plant chloroplasts are lens-shaped, usually 5-10 µm in diameter and 1-3 µm thick. Although leaves contain the majority of plants’ chloroplasts, they can also be found in stems, seeds, and un-ripened fruit. BioVision’s Chloroplast Isolation Kit provides an easy method to isolate chloroplast from a broad range of plant types including dicots, monocots, and conifers. Isolated chloroplasts can be used to study photosynthetic processes and as starting materials for chloroplast membrane, protein, chloroplast DNA and chloroplast RNA isolations
|#Cat + Size||K2027 (Size: 20 preparations)|
|Kit Summary||BioVision’s OrgFrontier™ Peroxisome Isolation Kit provides a proprietary set of reagents and buffers designed to enable the extraction and isolation of intact peroxisomes from mammalian tissues and cultured cells. The protocol requires an ultracentrifugation step and can be completed in one day.|
|Detection Method||Gradient Separation|
|Applications||Enrichment of peroxisome-associated proteins for western blot and ELISA, Peroxisome studies and peroxisome protein profiling, Isolation of peroxisomes from mammalian tissues and cultured cells|
|Features & Benefits||• Extraction and isolation of intact peroxisomes from mammalian tissues and cultured cells|
• OptiPrep™ Density Gradient Medium|
• Homogenization Buffer
• EZBlock™ Protease Inhibitor Cocktail
• Gradient Dilution Buffer
|Storage Conditions||Multiple Temperatures|
|Shipping Conditions||Gel Pack|
|USAGE||For Research Use Only! Not For Use in Humans.|
Should I apply brakes to the centrifuge during the density gradient separation of peroxisomes?
We recommend centrifuging without applying brakes for the isolation of peroxisomes to prevent remixing of the different phases. The protocol requires that acceleration & deceleration to be shut off completely so that the centrifuge accelerates slowly and high forces do not disturb the gradient.
For how long can we keep our peroxisomes in PBS (or other buffer) and at what temperature without affecting the peroxisome stability (storage conditions)?
If the peroxisomes are used the same day it is isolated, it can be kept at 4 °C or on ice. But if not, it should be aliquoted and stored at -20 °C.
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