Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can fuse with proteins of interest without interfering significantly with their assembly and function.
|Alternate Name||GFP Polyclonal Antibody, Anti-GFP antibody, GFP antibody, Anti-GFP, GFP, green fluorescence protein, EGFP, EBFP, EYFP, RFP|
|Formulation||In phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01 % thimerosal.|
|Species Reactivity||All species|
|Application||Western blot, Immunoprecipitation|
|Application & Usage||Western blotting (0.5-4 µg/ml) and in immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody reacts with GFP, and its variants EGFP, RFP, YFP, and CFP, etc. Recombinant EGFP (Cat.# 4999-100), RFP|
|Handling||The antibody solution should be gently mixed before use.|
|Storage Conditions||-20 °C|
|Shipping Conditions||Gel Pack|
|USAGE||For Research Use Only! Not For Use in Humans.|
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