Description
Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can fuse with proteins of interest without interfering significantly with their assembly and function.
Datasheet
| Antibody Target | GFP |
|---|---|
| Alternate Name | GFP Polyclonal Antibody, Anti-GFP antibody, GFP antibody, Anti-GFP, GFP, green fluorescence protein, EGFP, EBFP, EYFP, RFP |
| Host | Rabbit |
| Antibody Type | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | Recombinant EGFP |
| Accession # | ABG78037 |
| Gene ID | 110612126 |
| Appearance | Colorless liquid |
| Concentration | 0.5 mg/ml |
| Formulation | In phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01 % thimerosal. |
| Purification | Affinity purified |
| Species Reactivity | All species |
| Application | Western blot, Immunoprecipitation |
| Application & Usage | Western blotting (0.5-4 µg/ml) and in immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody reacts with GFP, and its variants EGFP, RFP, YFP, and CFP, etc. Recombinant EGFP (Cat.# 4999-100), RFP |
| Handling | The antibody solution should be gently mixed before use. |
| Storage Conditions | -20 °C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
FAQ
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CITATIONS
Jared P. May, RNA virus evasion of nonsense-mediated decay. PLoS Pathog, Nov 2018; 30452463.
Itoh, Eriko et al. (2017) Sirtuin E is a fungal global transcriptional regulator that determines the transition from the primary growth to the stationary phase, J Biol Chem. 2017 May 2
Lin, Ya-Hui et al. (2016) Rational design of a synthetic mammalian riboswitch as a ligand-responsive -1 ribosomal frame-shifting stimulator, Nucleic Acids Res. 2016 Oct 14;44(18):9005-9015.
Siljamaki, Elina et al. (2016) SPRED1 Interferes with K-ras but Not H-ras Membrane Anchorage and Signaling. Mol Cell Biol. 2016 Sep 26;36(20):2612-25.
Maus et al., Missense mutation in immunodeficient patients shows the multifunctional roles of coiled-coil domain 3 (CC3) in STIM1 activation. PNAS, May 2015; 112: 6206 - 6211.
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