EZblock™ Protease Inhibitor Cocktail
Datasheet (PDF) | Safety Data Sheets (MSDS)(PDF)
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Description
A cocktail of four broad specificity protease inhibitors that can provide maximal protection to prevent degradation of proteins in mammalian tissue and cell extracts by inhibition of various serine and cysteine proteases.
Datasheet
| Cat # +Size | K271-500 |
|---|---|
| Size | 1 kit |
| Appearance | Lyophilized solid |
| Solubility | DMSO |
| Handling | Protect from air, light and moisture |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
FAQ
What is the method of preperation to use this product?
This product is provided as a lyophilized powder. You have to reconstitute it in 250 µl of DMSO. This makes it 250 µl of 500X inhibitor cocktail. Which means that for every 499 or 500 µl of the lysate you can add 1 µl of the inhibitor. This means that the total amount of this inhibitor i.e., 250 µl is enough for 500 x 250 µl of the lysate, which is 125 ml of lysate. Therefore the dilution factor is 500.
Will DMSO is provided with the cocktail? While preparing 1X from 500X, what is used as a diluent (DMSO or water)
No, DMSO is not included with this cocktail. You need to procure anhydrous molecular biology gradient DMSO for this. The diluent used for the 500X to 1X dilution can be either the media, or the lysis solution/buffer or even PBS. It should not be DMSO.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
CITATIONS
Suseela Yelumalai, In vivo administration of quercetin ameliorates sperm oxidative stress, inflammation, preserves sperm morphology and functions in streptozotocin-nicotinamide induced adult male diabetic rats. Arch Med Sci., Dec 2018; 30697276.
Yasuhisa Ano, Iso-α-Acids, the Bitter Components of Beer, Suppress Microglial Inflammation in rTg4510 Tauopathy. Molecules, Nov 2018; 30501069.
Walter A. Baseler et al., Reversal of mitochondrial proteomic loss in Type 1 diabetic heart with overexpression of phospholipid hydroperoxide glutathione peroxidase. Am J Physiol Regulatory Integrative Comp Physiol, Apr 2013; 304: R553 - R565.
Makarand Deo et al., KCNJ2 mutation in short QT syndrome 3 results in atrial fibrillation and ventricular proarrhythmia. PNAS, Mar 2013; 110: 4291 - 4296.
Makarand Deo et al., KCNJ2 mutation in short QT syndrome 3 results in atrial fibrillation and ventricular proarrhythmia. PNAS, Feb 2013; 10.1073/pnas.1218154110.
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