The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process – simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 µl. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.

Technical Specifications
Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion > 99.5%
Elution Volume ≥ 10 µl
Equipment Thermocycler with heated lid and microcentrifuge.
Input 100 pg – 2 µg of DNA.
Processing Time 1.5 hours
Recovery > 80%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.



ZymoTaq DNA Polymerase has been specifically designed for use in bisulfite amplification reactions. ZymoTaq is available as a stand-alone polymerase (E2001/E2002), PreMix (E2003/E2004), and qPCR PreMix (E2054/E2055).

Leaving the desulphonation buffer on the column longer than recommended will cause more degradation and subsequently result in lower yields.

Bisulfite conversion will work regardless of context, so the kits are compatible with genomic DNA derived from plants and other species with high non-CpG methylation levels.

Following bisulfite treatment of genomic DNA, nonmethylated cytosine residues are converted into uracil. The recovered DNA is typically A, U, and T-rich. The original base-pairing no longer exists. Instead, it is single-stranded with limited non-specific base-pairing at room temperature. The absorption coefficient at 260 nm resembles that of RNA. Use a value of 40 μg/ml for Ab260 = 1.0 when determining the concentration of the recovered bisulfite-treated DNA. To visualize, run converted DNA on agarose gel then chill the gel on ice for 30 minutes. The expected smear will be between 100-1500bp.

Poor conversion efficiency and low yields can be due to a variety of different experiment-specific conditions. Please contact Technical Support to discuss your specific experimental conditions and further troubleshoot with a product specialist.





Mar 17 2021

4 review stars

More effective in less time.

Quick, easy and effective bisulfite modification system. This improved version that considerably saves incubation time, is a great success for lovers of epigenetics.

Application Area: Bisulfite conversion for DNA methylation analysis

Yang Zhao

Johns Hopkins Medicine

Mar 16 2021

4 review stars

Reliable products with consistent results.

EZ DNA Methylation-Lightning™ Kit is the bisulfite conversion kit we have been using for years. It appeared that this is one of the kits producing pure converted DNA and would not affect downstream applications such as digital PCR which requires high purity of DNA. It is a bead-based method, which is much easier to use than others. We use it for different starting concentrations from 100 pg to 2000 ng. We get consistent results from this kit all the time.

Application Area: Bisulfite conversion of human DNA

Rakel Tryggva

Johns Hopkins University

Mar 16 2021

5 review stars

High quality methylation results.

We experience very high-quality results with this product and find the results reproducible and very reliable. We have been using Zymo methylation kits for years now, first the gold kit and now we are mainly using the lighting kit. Tech support is always very helpful and responsive whenever we have questions.

Application Area: Whole genome sequencing data

Prat Gupta

CellMax Life

Dec 17 2020

5 review stars

Easy to use bisulphite conversion kit

The lightning kit is super-easy to use, efficient and reliable resource for bisulphite conversion. Great tool for methylation research.

Application Area: Methylation

Zohar Mukamel

Weizmann Institute of Science

Dec 05 2020

5 review stars

Great results, easy to use.

Easy to use, easy to scale down to single cell platforms and automation. Great results!

Application Area: Study DNA methylation during early development

Kit Components

Cat # Name Size
C1004-50 Zymo-Spin IC Columns 50 Pack
C1001-50 Collection Tubes 50 Pack
D5002-4 M-Wash Buffer 24 ml
D5001-4 M-Wash Buffer 6 ml
D5005-3 M-Binding Buffer 30 ml
D5002-6 M-Elution Buffer 4 ml
D5001-6 M-Elution Buffer 1 ml
D5006-3 M-Binding Buffer 125 ml
D5030-5 L-Desulphonation Buffer 10 ml
D5031-5 L-Desulphonation Buffer 40 ml
D5030-1 Lightning Conversion Reagent 1.5 ml

EZ DNA Methylation-Lightning Kit

Cat # Name Size
D5030-E EZ DNA Methylation-Lightning Kit (CE-IVD) 50 Rxns.
D5031-E EZ DNA Methylation-Lightning Kit (CE-IVD) 200 Rxns.
D5030T EZ DNA Methylation-Lightning Kit 10 Rxns.
D5030 EZ DNA Methylation-Lightning Kit 50 Rxns.
D5031 EZ DNA Methylation-Lightning Kit 200 Rxns.