BioVision’s Reducing Agent Compatible BCA Protein Assay Kit is the only protein estimation kit on the market that is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM & β-mercaptoethanol up to 35 mM. This kit is adapted from BioVision’s BCA Assay Kit II and is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in alkaline conditions. Reducing agents in samples interfere with BCA assay due to their ability to reduce Cu+2. BioVision’s Reducing Agent Compatible BCA Protein Assay Kit is useful for determing protein concentrations of samples containing DTT, TCEP & β-mercaptoethanol. The assay is linear over a wide range of protein concentration between 25-2000 μg/ml. In general, protein concentrations are calculated with reference to a commonly used protein standard. The kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.
|Cat # +Size||K818-1000|
|Detection Method||Absorbance (562 nm)|
|Applications||Measuring total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.|
|Features & Benefits||• Simple & convenient. • Optimized for determining total protein content of samples containing strong reducing agents such as DTT (10 mM), TCEP (20 mM) & β-mercaptoethanol (35 mM). • The assay is linear over a wide range of protein concentration (25-2000 μg/ml).|
|Kit Components||• BCA Reagent A • BCA Reagent B • BSA Standard (2 mg/ml) • Blocking Reagent (20 mg/tube) • Blocking Reagent Buffer|
|USAGE||For Research Use Only! Not For Use in Humans.|
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