- Includes pUC19MN primers in 20 µl
The Methylated & Non-methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers. The set is ideal as a “spike-in” control to assess bisulfite conversion efficiency within the same reaction as the sample, or to produce known mixtures of methylated and non-methylated DNA for assay calibration. The non-methylated pUC19 DNA is pUC19 isolated from a methylation-negative strain of bacteria (Dam–, Dcm–), and the methylated pUC19 DNA is pUC19 enzymatically methylated at all cytosines in the dinucleotide sequence 5’…CpG…3′ by CpG Methylase.
|pUC19 DNA has been linearized at position 2177 using ScaI endonuclease.
Methylated & Non-methylated pUC19 DNA Set