LSY-10007 Chloramphenicol(CAP) ELISA Test Kit (Tissue, egg)

LSY-10007 Chloramphenicol(CAP) ELISA Test Kit (Tissue, egg)

Manual (PDF)   |   MSDS (PDF)

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Catalog# LSY-10007


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Product Details

Test Principle

This test kit is based on theindirect competitive enzyme immunoassay for the detection of Chloramphenicol in the sample.The coupling antigenis pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicolantibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained.

Product Specifications



Sample type

Chicken, duck, pork, fish, shrimp, beef, lamb




15 ppt

Reaction mode

25℃, 45 min~15 min

Detection limit

Tissue(method 1) 7.5 ppt; Tissue(method 2) 15 ppt; Egg 15ppt

Cross reactivity

Chloramphenicol 100% ; Thiamphenicol< 0.1% ; Florfeniol< 0.1%

Sample recovery rate

Tissue, egg 95±25%

Incubation Temperature


Incubation Time


Kit Contains

  • Micro-well strips : 12 strips with 8 removable wells each
  • 7× standard solution (1mL each) : 0ppt, 15ppt, 45ppt, 135ppt,405ppt, 1215ppt, 10ppb
  • Enzyme conjugate : 7ml (red cap)
  • Antibody working solution: 7ml (blue cap)
  • Substrate A : 7ml (white cap)
  • Substrate B: 7ml (black cap)
  • Stop solution: 7ml (yellow cap)
  • 20× concentrated washing buffer: 40ml (white cap)
  • 2×concentrated redissolving solution: 50ml (transparent cap)


store at2-8 ℃, not frozen.

Expiry date

12 months; date of production is on the box.

Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.

*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Other materials required but not supplied

1) Equipments:microplate reader,homogenizer, nitrogen-drying device,vortex, centrifuge, measuring pipets, andbalance( asensibility reciprocalof0.01 g), incubator.
2) Micropipettors: single-channel 20-200 µL, 100-1000 µL, andmulti-channel 30~300 μl.
3) Reagents: Ethyl acetate,N-hexane, NaOH, K2HPO4·3H2O, HCl.


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