LSY-10007 Chloramphenicol(CAP) ELISA Test Kit (Tissue, egg)

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LSY-10007 Chloramphenicol(CAP) ELISA Test Kit (Tissue, egg)


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Catalog# LSY-10007

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Product Details




Test Principle


This test kit is based on theindirect competitive enzyme immunoassay for the detection of Chloramphenicol in the sample.The coupling antigenis pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicolantibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained.


Product Specifications


Synonyms

-

Sample type

Chicken, duck, pork, fish, shrimp, beef, lamb

Reactivity

Universal

Sensitivity

15 ppt

Reaction mode

25℃, 45 min~15 min

Detection limit

Tissue(method 1) 7.5 ppt; Tissue(method 2) 15 ppt; Egg 15ppt

Cross reactivity

Chloramphenicol 100% ; Thiamphenicol< 0.1% ; Florfeniol< 0.1%

Sample recovery rate

Tissue, egg 95±25%

Incubation Temperature

25℃

Incubation Time

30min～15min

Kit Contains

  • Micro-well strips : 12 strips with 8 removable wells each
  • 7× standard solution (1mL each) : 0ppt, 15ppt, 45ppt, 135ppt,405ppt, 1215ppt, 10ppb
  • Enzyme conjugate : 7ml (red cap)
  • Antibody working solution: 7ml (blue cap)
  • Substrate A : 7ml (white cap)
  • Substrate B: 7ml (black cap)
  • Stop solution: 7ml (yellow cap)
  • 20× concentrated washing buffer: 40ml (white cap)
  • 2×concentrated redissolving solution: 50ml (transparent cap)

Storage

store at2-8 ℃, not frozen.

Expiry date

12 months; date of production is on the box.

Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.

*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.







Other materials required but not supplied



1) Equipments:microplate reader,homogenizer, nitrogen-drying device,vortex, centrifuge, measuring pipets, andbalance( asensibility reciprocalof0.01 g), incubator.
2) Micropipettors: single-channel 20-200 µL, 100-1000 µL, andmulti-channel 30～300 μl.
3) Reagents: Ethyl acetate,N-hexane, NaOH, K2HPO4·3H2O, HCl.













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