Human Nuclear Mitotic Apparatus Protein 1, NUMA1 ELISA Kit
Product Summary
• Size: 48T,96T
• Sensitivity: 0.022ng/ml
• Detection range: 0.05-20ng/ml
• Reactive with: Human
Product Details
Product Specifications |
|
---|---|
Full Product Name: |
Nuclear Mitotic Apparatus Protein 1 |
Catalog No.: |
BL2971EH |
Size: |
48T,96T |
Detaction Range: |
0.05-20ng/ml |
Sensitivity: |
0.022ng/ml |
Sample Type: |
Serum, plasma, cell culture supernates |
Assay Type: |
Sandwich |
Species: |
Human |
Storage: |
2-8ºC |
Assay Time: |
1h 30m |
Background: |
Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division (PubMed: 7769006, PubMed: 17172455, PubMed: 19255246, PubMed: 24996901, PubMed: 26195665, PubMed: 27462074). Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles (PubMed: 12445386, PubMed: 11956313). Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner (PubMed: 23870127, PubMed: 24109598, PubMed: 24996901, PubMed: 26765568). In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle (PubMed: 23027904, PubMed: 22327364, PubMed: 23921553). During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation (PubMed: 22327364, PubMed: 23921553, PubMed: 24996901, PubMed: 24371089). Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro (PubMed: 24996901, PubMed: 24371089). Also required for proper orientation of the mitotic spindle during asymmetric cell divisions (PubMed: 21816348). Plays a role in mitotic MT aster assembly (PubMed: 11163243, PubMed: 11229403, PubMed: 12445386). Involved in anastral spindle assembly (PubMed: 25657325). Positively regulates TNKS protein localization to spindle poles in mitosis (PubMed: 16076287). Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume (PubMed: 10075938). Required for epidermal differentiation and hair follicle morphogenesis (By similarity). |
UniProt: |
|
MASS(Da): |
238,260 |
GeneID: |
4926 |
Synonyms: |
NMP-22;Nuclear matrix protein-22;Nuclear mitotic apparatus protein;Nuclear mitotic apparatus protein 1;NUMA 1;NuMA protein;NUMA1;SP-H antigen |
Gene Names: |
NUMA1 |
Research Area: |
Cell Biology |
Target Protein: |
NUMA1 |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Components
Components | Quantity |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate | (25x) 20ml x1 |
Biotinylated antibody | 1ml x1 |
Documents
Technical Guide
Technical Guide: Human Nuclear Mitotic Apparatus Protein 1, NUMA1 ELISA KitSafety Data Sheets (SDS)
MSDS: Human Nuclear Mitotic Apparatus Protein 1, NUMA1 ELISA KitReferences
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