Human Caspase-4, CASP4 ELISA Kit
Product Summary
• Size: 48T,96T
• Sensitivity: 0.022ng/mL
• Detection range: 0.05-20ng/mL
• Reactive with: Human
Product Details
Product Specifications |
|
---|---|
Full Product Name: |
Caspase-4 |
Catalog No.: |
BL0739EH |
Size: |
48T,96T |
Detaction Range: |
0.05-20ng/mL |
Sensitivity: |
0.022ng/mL |
Sample Type: |
Serum, plasma, cell culture supernates |
Assay Type: |
Sandwich |
Species: |
Human |
Storage: |
2-8ºC |
Assay Time: |
1h 30m |
Background: |
Inflammatory caspase that acts as an essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS (PubMed: 23516580, PubMed: 24879791, PubMed: 25119034, PubMed: 22246630, PubMed: 26174085, PubMed: 26173988, PubMed: 26508369, PubMed: 25964352). Thiol protease that cleaves a tetrapeptide after an Asp residue at position P1 (PubMed: 7797510, PubMed: 23516580). Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, followed by IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed: 26375003, PubMed: 32109412). Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection: in later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which catalyzes cleavage of GSDMD, resulting in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion (PubMed: 25121752, PubMed: 26375003, PubMed: 25964352, PubMed: 32109412). Cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed: 32109412). Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation (PubMed: 25121752, PubMed: 26375003, PubMed: 25964352). Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity (PubMed: 26508369). Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP Peptides found Alzheimer's patient brains (PubMed: 15123740, PubMed: 22246630, PubMed: 23661706). Activated by direct binding to LPS without the need of an upstream sensor (PubMed: 25119034). Does not directly process IL1B (PubMed: 7743998, PubMed: 7797510, PubMed: 7797592). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed: 28314590). |
UniProt: |
|
MASS(Da): |
43,262 |
GeneID: |
837 |
Synonyms: |
CASP4;CASP-4;Caspase-4;Caspase-4 subunit p10;Caspase-4 subunit p20;ICE and Ced-3 homolog 2;ICE(rel-II;ICH-2;Mih1;Protease TX |
Gene Names: |
CASP4 |
Research Area: |
Others |
Target Protein: |
CASP4 |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Components
Components | Quantity |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate | (25x) 20ml x1 |
Biotinylated antibody | 1ml x1 |
Documents
Technical Guide
Technical Guide: Human Caspase-4, CASP4 ELISA KitSafety Data Sheets (SDS)
MSDS: Human Caspase-4, CASP4 ELISA KitReferences
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