Human 1, 25-Hydroxyvitamin D,1, 25-(OH)D, CYP24A1 ELISA Kit
Product Summary
• Size: 48T,96T
• Sensitivity: 3.14pmol/L
• Detection range: 7-1500pmol/L
• Reactive with: Human
Product Details
Product Specifications |
|
---|---|
Full Product Name: |
1,25-Hydroxyvitamin D |
Catalog No.: |
BL1919EH |
Size: |
48T,96T |
Detaction Range: |
7-1500pmol/L |
Sensitivity: |
3.14pmol/L |
Sample Type: |
Serum, plasma, cell culture supernates |
Assay Type: |
Sandwich |
Species: |
Human |
Storage: |
2-8ºC |
Assay Time: |
1h 30m |
Background: |
A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)) (PubMed: 24893882, PubMed: 15574355, PubMed: 8679605, PubMed: 11012668, PubMed: 16617161, PubMed: 29461981). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid (PubMed: 24893882, PubMed: 15574355). With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product (PubMed: 11012668, PubMed: 8679605). Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol (PubMed: 25727742). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed: 8679605). |
UniProt: |
|
MASS(Da): |
58,875 |
GeneID: |
1591 |
Synonyms: |
1,25-dihydroxyvitamin D,3 24-hydroxylase, mitochondrial;24-OHase;CYP 24A1;CYP24A1;Cytochrome P450 24A1;Cytochrome P450-CC24;Vitamin D, 3 24-hydroxylase |
Gene Names: |
CYP24A1 |
Research Area: |
Others |
Target Protein: |
CYP24A1 |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Components
Components | Quantity |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate | (25x) 20ml x1 |
Biotinylated antibody | 1ml x1 |
Documents
Technical Guide
Technical Guide: Human 1, 25-Hydroxyvitamin D,1, 25-(OH)D, CYP24A1 ELISA KitSafety Data Sheets (SDS)
MSDS: Human 1, 25-Hydroxyvitamin D,1, 25-(OH)D, CYP24A1 ELISA KitReferences
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