Bovine Ubiquitin-like Protein Isg15, ISG15 ELISA Kit
Product Summary
• Size: 48T,96T
• Sensitivity: 2.45ng/L
• Detection range: 5-2000ng/L
• Reactive with: Bovine
Product Details
Product Specifications |
|
---|---|
Full Product Name: |
Ubiquitin-like protein ISG15 |
Catalog No.: |
BL0359E |
Size: |
48T,96T |
Detaction Range: |
5-2000ng/L |
Sensitivity: |
2.45ng/L |
Sample Type: |
Serum, plasma, cell culture supernates |
Assay Type: |
Sandwich |
Species: |
Bovine |
Storage: |
2-8ºC |
Assay Time: |
1h 30m |
Background: |
Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Exhibits antiviral activity towards both DNA and RNA viruses. The secreted form of ISG15 can: induce natural killer cell proliferation, augment lymphokine-activated-killer (LAK) activity, induce dendritic cell maturation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity (By similarity). The secreted form acts through the integrin ITGAL/ITGB2 receptor to initiate activation of SRC family tyrosine kinases including LYN, HCK and FGR which leads to secretion of IFNG and IL10; the interaction is mediated by ITGAL (By similarity). In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy (PubMed:9546718). |
UniProt: |
|
MASS(Da): |
17,310 |
GeneID: |
281871 |
Synonyms: |
BoUCRP;Interferon-stimulated gene product 17;ISG15;Ubiquitin cross-reactive protein;Ubiquitin-like protein ISG15 |
Gene Names: |
ISG15 |
Research Area: |
Others |
Target Protein: |
ISG15 |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Components
Components | Quantity |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate | (25x) 20ml x1 |
Biotinylated antibody | 1ml x1 |
Documents
Technical Guide
Technical Guide: Bovine Ubiquitin-like Protein Isg15, ISG15 ELISA KitSafety Data Sheets (SDS)
MSDS: Bovine Ubiquitin-like Protein Isg15, ISG15 ELISA KitReferences
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