Zyppy-96 Plasmid Kit

Pellet-free, high-quality plasmid DNA in only 8 minutes


  • Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
  • High Quality: Ready for PCR, sequencing, cloning, and transfection.
  • Streamlined Protocol: Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.


The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.

Technical Specifications

Applicable For Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
Elution Volume ≥ 30 µl
Equipment Centrifuge with micro plate carriers
Processing Time 45 min
Purity Typical Abs 260/280 ≥ 1.8.
Size Range Up to 25 kb
Yield Up to 10 µg per preparation depending on the plasmid copy number, culture growth conditions, and strain of E. coli processed.

Product FAQ

Q1: Can E.coli grown in enriched growth media be used with this kit?

Q2: What is the composition of the Zyppy Elution buffer?

Q3: Can the purified plasmid be transfected into eukaryotic cell lines?

Q4: Can the Zyppy Plasmid kits be used to isolate large constructs (BAC/PAC)?

Q5: What are the endotoxin levels in plasmid DNA isolated with the Zyppy Kits?

Q6: I accidentally left my Neutralization Buffer at room temperature. Will it be okay?

Q7: I ran out of Zyppy Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?


Kit Components

Zyppy-96 Plasmid Kit


Cat # Name Size
D4041 Zyppy-96 Plasmid Kit 2 x 96 Preps
D4042 Zyppy-96 Plasmid Kit 4 x 96 Preps
D4043 Zyppy-96 Plasmid Kit 8 x 96 Preps