- Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
- High Quality: Ready for PCR, sequencing, cloning, and transfection.
- Streamlined Protocol: Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.
The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.
|Applicable For||Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.|
|Elution Volume||≥ 30 µl|
|Equipment||Centrifuge with micro plate carriers|
|Processing Time||45 min|
|Purity||Typical Abs 260/280 ≥ 1.8.|
|Size Range||Up to 25 kb|
|Yield||Up to 10 µg per preparation depending on the plasmid copy number, culture growth conditions, and strain of E. coli processed.|
Q1: Can E.coli grown in enriched growth media be used with this kit?
Q2: What is the composition of the Zyppy Elution buffer?
Q3: Can the purified plasmid be transfected into eukaryotic cell lines?
Q4: Can the Zyppy Plasmid kits be used to isolate large constructs (BAC/PAC)?
Q5: What are the endotoxin levels in plasmid DNA isolated with the Zyppy Kits?
Q6: I accidentally left my Neutralization Buffer at room temperature. Will it be okay?
Q7: I ran out of Zyppy Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Zyppy-96 Plasmid Kit