- Simple: Quickly and easily rescue plasmid from yeast.
- Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
- High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
The Zymoprep-96 Yeast Plasmid Miniprep kit provides all the necessary reagents for high-throughput plasmid isolation from S. cerevisiae, C. albicans, S. pombe and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The Zymoprep-96 is a simple and efficient yeast plasmid miniprep kit that is based on the classic E. coli alkaline lysis method with our Zymolyase added in the first solution. There is no need for glass beads or phenol. The Zymoprep-96 incorporates our Zymoprep II kit into a 96-well format resulting in a high-throughput method that recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard-to-isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.
|Applicable For||Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications|
|Elution Volume||≥ 10 µl per well|
|Equipment||Centrifuge with microplate carriers|
|Processing Volume||≤ 1.5 ml of Culture|
|Sample Source||Cell Culture (Colonies/Patches or Liquid Culture)|
|Size Range||Up to 25 kb.|
|Yield||Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures|
Q1: What is the difference between Zymoprep Yeast Plasmid Miniprep I and Miniprep II?
Q2: What is the typical plasmid yield?
Q3: Can this kit be used to isolate linear plasmid DNA?
Q4: If I’m using stationary phase yeast cells, what can I do to improve sample lysis?
Q5: What yeast strains are these kits compatible with?
Zymoprep-96 Yeast Plasmid Miniprep