- Quick recovery of ultra-pure DNA from agarose gels.
- Column design permits DNA elution at high concentrations into minimal volumes.
- Eluted DNA is well-suited for use in DNA ligation, sequencing, labeling, PCR, etc.
|Next Generation sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions.
|≥ 6 µl of DNA Elution Buffer
|A260/A280 >1.8, A260/A230>1.8
|DNA excised from TAE/TBE-buffered agarose gels.
|50 bp to 23 kb
|Typically, up to 5 µg total DNA per column can be eluted with ≥6 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%
Q1: What is the difference between capped and uncapped?
Q2: Is it possible to use higher binding capacity columns for the Zymoclean workflow?
Q3: Are the columns and DNA Wash buffer interchangeable with the DCC-5 Kit?
Q4: Should more Agarose Dissolving Buffer be used for higher % gels?
Q5: Can Zymoclean be used for samples in solution (without agarose gel)?
Q6: Can Zymoclean be used to recover ssDNA?
Q7: What is the maximum weight of gel slice that can be used?
“The best advantage about this kit is that it is really quick to make it, and the total DNA recuperated have a good quality and it have a good concentration to follow the experiments.”
– Ingrid M.
“The kit was very easy to follow and yielded good results. It was more affordable than the Qiagen Gel Extraction kits, but worked just as efficiently. Solid product“
– Thomas B (University of Tennessee, Knoxville)
“Protocols are very well written and Instructions easy to follow. Always had good results with your products.”
– Simin H. (University of California, Irvine)
“I had tried three other kits to do a certain recovery before this, I was getting bad yields, the elution volume required was quite large, the protocols were not as easy to follow and they took longer to complete. I liked everything about this kit, it is simple, efficient and quick. The protocol is easy to follow and the yield was 4-5 times better than what I got from other kits.”
– Wendy P. (University of Auckland)
“Quick story. I’ve been having trouble cleaning up PCR products for sequencing. My gel bands look beautiful and bright, but after clean-up with Millipore clean up kits, I was left with between 1 and 3 nanograms of DNA per ul of eluted product. I did over 100 clean-ups this way without any improvement. I just did 17 clean-ups using a colleague’s ZymoResearch spin columns and each clean-up was fantastic. Your kits are also less expensive per reaction. You’ve just made a customer for life.”
– Jeremey R, Francis Marion University
“The ADB buffer and higher temperature used melted the gel quicker than previously used kits thus making DNA recovery using this kit more efficient and generally quicker than previously used kits.”
– Kelly-Anne F.
Zymoclean Gel DNA Recovery Kit