Highlights
- High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
- Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
- Unique column design: Zero buffer retention and low (30 µl) elution volume.
Description
Technical Specifications
| Applicable For | Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, transfections, and other sensitive applications. |
|---|---|
| Elution Volume | ≥ 30 µl |
| Endotoxin Levels | < 50 EU/µg |
| Equipment | Microcentrifuge |
| Processing Time | 15 min |
| Purity | Typical Abs260/280 ≥1.8. |
| Size Range | Up to 25 kb |
| Yield | Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. |
Product FAQ
Q1: What is the difference between the ZymoPURE Miniprep Kit and the ZR Plasmid Miniprep – Classic?
The ZymoPURE Miniprep kit utilizes a modified alkaline lysis system in conjunction with a novel binding system. This results in higher yields, higher concentrations, and lower endotoxin levels when compared to the ZR Plasmid Miniprep-Classic.
Q2: Can the kit be used to isolate large constructs (BAC/PAC)?
No, we recommend using our ZR BAC DNA Miniprep Kit (D4048) or ZymoPURE Miniprep Kit for isolating large constructs.
Q3: Can the purified plasmid be transfected into eukaryotic cell lines (i.e. Is it transfection grade)?
Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.
Q4: What is the composition of the DNA Elution buffer?
10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.
Q5: Can I perform the procedure using a vacuum manifold?
Yes, but we recommend performing a dry spin in the microcentrifuge after the last wash step, prior to the elution step.
Q6: I ran out of Plasmid Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Plasmid Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Zyppy Wash Buffer, or 80% ethanol.
Q7: What are the endotoxin levels in plasmid DNA isolated using the ZR-Plasmid Miniprep Classic Kit?
= 50 Endotoxin Units/µg of plasmid DNA.
Citations
Kit Components
| Cat # | Name | Size |
|---|---|---|
|
D3004-4-16 |
DNA Elution Buffer | 16 ml |
|
D3004-4-4 |
DNA Elution Buffer | 4 ml |
|
D4027-1-160 |
Buffer P1 (Red) | 160 ml |
|
D4027-1-20 |
Buffer P1 (Red) | 20 ml |
|
D4027-1-80 |
Buffer P1 (Red) | 80 ml |
|
D4027-2-160 |
Buffer P2 (Green) | 160 ml |
|
D4027-2-20 |
Buffer P2 (Green) | 20 ml |
|
D4027-2-80 |
Buffer P2 (Green) | 80 ml |
|
D4027-3-220 |
Buffer P3 (Yellow) | 220 ml |
|
D4027-3-50 |
Buffer P3 (Yellow) | 50 ml |
|
D4027-4-24 |
Plasmid Wash Buffer | 24 ml |
|
D4027-4-48 |
Plasmid Wash Buffer | 48 ml |
|
D4027-4-6 |
Plasmid Wash Buffer | 6 ml |
|
D4036-3-30 |
Endo-Wash Buffer | 30 ml |
|
D4036-3-60 |
Endo-Wash Buffer | 60 ml |
|
C1001-50 |
Collection Tubes | 50 Pack |
|
C1019-50 |
Zymo-Spin IIN Columns | 50 Pack |
ZR Plasmid Miniprep – Classic
D4054/D4015/D4016
| Cat # | Name | Size |
|---|
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