Quick-DNA Urine Kit

Quick-DNA Urine Kit


  • Purify cellular and/or cell-free DNA easily and reliably from up to 40 ml of urine.
  • Uniquely formulated urine conditioning reagent allows stabilization of DNA in urine for up to 1 month at ambient temperature.
  • Zymo-Spin column technology ensures DNA is ready for all sensitive downstream applications including qPCR, DNA sequencing, arrays, and DNA methylation analysis.


The Quick-DNA Urine Kit is an innovative product designed for easy, reliable, and rapid isolation of cellular and/or cell-free DNA from up to 40 ml of urine. The product features a uniquely formulated urine DNA stabilization reagent that also functions as a precipitation reagent. After collection, total (cellular and cell-free) or cell-free urine can be stored at ambient temperature for up to one month by adding the Urine Conditioning Buffer. When ready to extract the urine DNA, just add the Clearing Beads, vortex, and centrifuge to collect the precipitate. Following precipitation, chemical lysis and enzymatic digestion are used to extract DNA from the precipitate. The DNA is subsequently purified and concentrated using Zymo-Spin IC-S Columns. Urine DNA isolated with the Quick-DNA Urine Kit is ideal for qPCR, array, methylation analysis, and other downstream applications.

Technical Specifications

Applicable For All sensitive downstream applications such as qPCR and Next-Generation sequencing.
Equipment Centrifuge, microcentrifuge, and heat block/bath.
Processing Volume ≤ 40 ml
Purity Typical A260/A280 ≥ 1.8
Sample Source Urine
Sample Storage Eluted DNA should be stored at ≤ -20°C.
Size Range 100 bp – 23 kb
Type Total DNA including cfDNA
Yield Up to 5 µg total DNA can be eluted into ≥ 10 µl. 6 – 1000 ng/ml for healthy females. 2 – 20 ng/ml for healthy males.

Product FAQ

Q1: If I plan to process my urine samples immediately, is the Urine Conditioning Buffer necessary?

Q2: What is the function of the Clearing Beads?

Q3: Can I process urine samples stabilized in DNA/RNA Shield?

Q4: What should I do if I see an oil layer on top of the pellet, after centrifugation?

Q5: Can cell-free DNA be isolated from urine stored at – 80 °C?

Q6: Is the Quick-DNA Urine kit compatible with DNA Urine Preserve from Streck?



Good yields of DNA in a very simple efficient process.

– R. W. (Medical University of South Carolina)

The kit is very useful as it lets us to analyze big volumes without an ultracentrifugation step.

– S. B.M. (University of Barcelona)

The protocol was easy to use/follow and it didn’t take a great deal of time to get results.

– S. H. (South College School of Pharmacy)

We tested it this week and it worked beautifully. We really would like to order this kit to process more samples!

– R. D. (Medical College of Wisconsin)

The Zymo Kit and more specifically the Cell-DNA method presents as the best option to improve the outcome of our LR-PCR method.

– R. D. (University of Sydney and Royal North Shore Hospital)

Urine DNA isolation kit is very much needed and I like Zymo kits for the simplicity an reliability.

– A. C. (Sanford-Burnham Medical Research Institute)

Kit Components

Quick-DNA Urine Kit


Cat # Name Size
D3061 Quick-DNA Urine Kit 50 Preps