- Quick & Easy: Simple 20 minute procedure.
- Highest Yield: Recover 3x more DNA.
- Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.
|Elution Volume||≥ 35 µl|
|Equipment||Water bath or heat block (55°C), microcentrifuge, and vortex.|
|Purity||High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230≥ 2.0).|
|Size Range||Capable of recovering genomic and mitochondrial DNA sized fragments > 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered.|
|Workflow||Utilize a Proteinase K Digestion and Zymo-Spin Column for effective recovery of DNA.|
|Yield||The DNA binding capacity of the column is 25 µg. Typically, mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 µg DNA per mg liver and kidney. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled.
Q1: What is the difference between the digestion buffers? Can one buffer be used for all sample types?
Q2: What is the difference between Quick-DNA and Quick-DNA Plus kits?
Q3: Can Proteinase K digestion be performed overnight in DNA/RNA Shield?
Q4: I’m seeing some yield inconsistencies with my blood samples, what’s happening?
Q5: Can the Quick-DNA Plus kit be used with bacterial samples?
Q6: Can I use Quick-DNA Plus to clean-up previously isolated DNA?
Q7: What is the purpose of adding beta-mercaptoethanol? Can this step be substituted or omitted?
Q8: Can Quick-DNA process crude lysates?
This kit is easy to use and provides a high quality yield.
– M.B. (Syracuse University)
“I was very impressed with the purity of the DNA.”
– C.V. (UCLA)
“Improved yield than Bioline and Qiagen Kits.”
– F.C. (Manchester Metropolitan University)
Quick-DNA Miniprep Plus Kit