- Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including those from humans, birds, rats, mice, cattle, etc.
- Ultra-high density BashingBeads are fracture resistant and chemically inert.
- Zymo-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.
The Quick-DNA Fecal/Soil Microbe Microprep Kit is a fecal and soil DNA extraction kit designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.
|All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
|≥ 20 µl
|Microcentrifuge, vortex, cell disruptor/pulverizer
|≤50 mg feces, ≤ 250 mg soil, ≤20 mg fungal/bacterial cells (wet weight)
|A260/A280 nm ≥1.8.
|Feces or soil
|DNA stored at ≤ -20°C.
|Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
|≤ 5 µg total DNA
Q1: My lysate seems viscous. What is causing this to happen? How can I fix this?
Q2: Are there any tips in optimzing bead beating conditions?
Q3: What is the purpose of Zymo-Spin II-µHRC step?
Q4: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?
Q5: When can an RNase A treatment be implemented in the protocol?
Quick-DNA Fecal/Soil Microbe Microprep