- Rapid, high-throughput method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including those from humans, birds, rats, mice, cattle, etc.
- Ultra-high density BashingBeads are fracture resistant and chemically inert.
- Zymo MagBinding Bead technology effectively removes PCR inhibitors from the DNA product.
The Quick-DNA Fecal/Soil Microbe 96 Magbead Kits are fecal and soil DNA extraction kits designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in as little as 90 minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo MagBinding Bead technology, which features Zymo Research’s Inhibitor Removal technology, is then used to isolate the DNA. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.
|Applicable For||All sensitive downstream applications such as qPCR and Next-Generation Sequencing.|
|Elution Volume||≥ 37.5 µl|
|Equipment||Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block, 2 mL 96 well plates and reagent carriers (user supplied).|
|Purity||A260/A280 nm ≥1.8.|
|Sample Source||Feces or soil|
|Sample Storage||DNA stored at ≤ -20°C.|
|Size Range||Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.|
|Yield||≤ 25 µg total DNA|
Q1: Can I use more sample input by scaling up the protocol?
Q2: Do you have scripts available for your automated kits and/or do you provide scripting support?
Q3: Are there any tips in optimzing bead beating conditions?
Q4: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?
Q5: When can an RNase A treatment be implemented in the protocol?
Quick-DNA Fecal/Soil Microbe 96 Magbead Kit