Since this kit is so sensitive, avoiding possible sources of contamination is key for a successful qPCR.
– Work in a clean environment and decontaminate the benchtop and pipettes with a 10% bleach solution.
– Wipe down the areas with ethanol to remove the trace amounts of bleach and allow the area to try.
– Use filtered tips.
– Take care to ensure that pipetting is efficient and accurate. Calibrate your pipettes regularly.
– Add standards directly into the premix and pipet up and down to flush out any remaining liquid from the tip.
– Avoid pipetting onto the walls as this may skew the results.
– The SYTO9 dye present within the premix is sensitive to light, so working quickly and diligently is recommended.

Our qPCR Premixes does not contain ROX Reference Dye. Ensure that the ROX channel of your device is switched off.

Our qPCR Premixes contains Syto9. You can use the SYBR Green channel for signal detection.

No, kits are not certified.

Primers are ITS1-F and ITS2-R.
Forward Primer: ITS-1F
5′- CTTGGTCATTTAGAGGAAGTAA-3′
Reverse Primer: ITS-2R
5′- GCTGCGTTCTTCATCGATGC-3′

They are based on this publication:
White, Thomas J., et al. “Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.” PCR protocols: a guide to methods and applications 18 (1990): 315-322

The kit targets the ITS region

The DNA is derived from S. cerevisiae strain TMY18.