- Quantify as little 20 femtograms of bacterial DNA in as little as 1 µl of sample.
- High specificity and sensitivity for bacterial DNA in a background of non-target DNA.
- Fast and simple: add samples to the PreMix… and quantify.
The Femto Bacterial DNA Quantification Kit can be used to detect and quantify bacterial DNA with high specificity and sensitivity. Bacterial DNA can be reliably quantified in a background of non-bacterial DNA such as fungal, animal, and plant DNA, etc. This is essential for downstream applications that require accurate DNA input including quantifying bacteria DNA template for Next-generation sequencing library preparation and metagenomic analysis amongst others. With the Femto Bacterial DNA Quantification Kit, one can dependably quantify as little as 20 fg DNA from 1 µl purified from biological liquids, bacterial cultures, or environmental DNA samples.
|Detection Range||20 fg to 20 ng DNA|
|Equipment||Real-time quantitative PCR system,vortex, microcentrifuge, PCR tube strip or PCR plate, optically transparent sealing film for PCR plate or tube strip caps|
|Sample Source||Purified DNA|
Q1: Is there any general advice on GLP for qPCR?
Q2: Does qPCR Premix contain ROX?
Q3: Which settings can be used for signal detection?
Q4: Are the Femto kits certified for low bioburden?
Q5: What is the origin of the bacterial DNA Standards?
Q6: What is strain JM109?
Q7: How is the copy number of the 16S rRNA gene calculated?
Q8: How to calculate the amount of genomic copies detected with E2006?
Femto Bacterial DNA Quantification Kit