Direct-zol RNA Kits

Direct-zol RNA Kits

The Direct-zol RNA Kits are RNA purification kits that provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. With these RNA purification kits, total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. These RNA purification kits use the Direct-zol™ method which assures unbiased recovery of small RNAs including miRNA (see below). The procedure for these RNA purification kits is easy: simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. With these RNA purification kits, no phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). These RNA purification kits allow the entire process to be complete in only 7 minutes.

* Direct-zol RNA kits facilitate rapid viral detection in TRIzol treated plasma samples of Wuhan patients:
Prof. Chaolin Huang, MD et al, Clinical features of patients infects with 2019 novel coronavirus in Wuhan, China, Jan. 24, 2020

The Direct-zol RNA method eliminates traditional phase separation and precipitation steps. RNA is isolated in as little as 7 minutes. Just add ethanol to any sample in TRIzol, bind, wash, and elute NGS-ready RNA.

Comparison Table

Catalog # Product Binding Capacity Elution Volume Format Cells Tissue
R2061 Direct-zol RNA Microprep 10μg 6 μl Spin-Column ≤ 1×106 Cells ≤ 5 mg Tissue
R2051 Direct-zol RNA Miniprep 50 μg 25 μl Spin-Column ≤ 5×106 Cells ≤ 25 mg Tissue
R2071 Direct-zol RNA Miniprep Plus 100 μg 50 μl Spin-Column ≤ 1×107 Cells ≤ 50 mg Tissue
R2055 Direct-zol-96 10μg 10 μl 96-Well Plate ≤ 1×106 Cells ≤ 5 mg Tissue
R2101 Direct-zol-96 MagBead 10μg 50 μl Magnetic Beads ≤ 1×106 Cells ≤ 5 mg Tissue