WNT5B Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM
- Reactivity: Human
- Predicted Reactivity:
-
Datasheet
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IMAGES
Flow cytometric analysis of WNT5B was done on LOVO cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54708R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
WNT5B Monoclonal Antibody |
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CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic Peptide within C terminal human Wnt5b. |
| IMMUNOGEN RANGE |
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| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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| GENE ID |
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| SUBCELLULAR LOCATION |
Extracellular matrix |
| SYNONYMS |
MGC2648 antibody, Protein Wnt-5b antibody, Wingless type MMTV integration site family, member 5B antibody, WNT 5B antibody, WNT 5B protein antibody, Wnt family member 5B antibody, Wnt5b antibody, WNT5B protein antibody, WNT5B_HUMAN antibody |
| BACKGROUND |
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008] |
| APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100) |