Tyrosine Hydroxylase Polyclonal Antibody
- Size: 100ul
- Application: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
- Reactivity: Human, Mouse, Rat, Others
- Predicted Reactivity: Dog, Cow
-
Datasheet
Tech Support
IMAGES
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated (bs-0016R) at 1:400 overnight at 4°C, DAB staining.
Human PC-3 lysates probed with Tyrosine Hydroxylase Polyclonal Antibody, unconjugated (bs-0016R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated (bs-0016R) at 1:200 overnight at 4°C, DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Tyrosine Hydroxylase(Neuronal Marker)/Tyk2/TYH Polyclonal Antibody, Unconjugated (bs-0016R) 1:200 followed by conjugation to the secondary antibody and DAB stainingn
RSC96 probed with Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated (bs-0016R) at 6ug for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
PRODUCT DETAILS
SPECIFICATIONS |
|
|---|---|
|
PRODUCT NAME |
Tyrosine Hydroxylase Polyclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
KLH conjugated synthetic peptide derived from human Tyrosine Hydroxylase |
| IMMUNOGEN RANGE |
101-165/528 |
| CLONALITY |
Polyclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
|
|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Cytoplasm, Cell membrane |
| SYNONYMS |
TYH; DYT14; DYT5b; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH |
| BACKGROUND |
Neuronal Marker Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease. |
| APPLICATION DILUTION |
WB(1:300-1000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200) |
