TEF1 Monoclonal Antibody
- Size: 100ul
- Application: WB, IHC-P, IF(ICC), IHC
- Reactivity: Human, Mouse, Rat
- Predicted Reactivity:
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Datasheet
Tech Support
IMAGES
MG-63 cell;4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TEF1 ) monoclonal Antibody, Unconjugated (bsm-54758R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
A549 cell;4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TEF1 ) monoclonal Antibody, Unconjugated (bsm-54758R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Siha cell;4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TEF1 ) monoclonal Antibody, Unconjugated (bsm-54758R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TEF1 Monoclonal Antibody, Unconjugated (bsm-54758R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TEF1 Monoclonal Antibody, Unconjugated (bsm-54758R) at 1:200 for 30 minutes at room temperature, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
TEF1 Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic Peptide within N-terminal Human TEF1. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
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| SUBCELLULAR LOCATION |
Nucleus |
| SYNONYMS |
AA antibody, Atrophia areata peripapillary chorioretinal degeneration antibody, NTEF 1 antibody, NTEF-1 antibody, NTEF1 antibody, Protein GT IIC antibody, Protein GT-IIC antibody, REF 1 antibody, REF1 antibody, SV40 transcriptional enhancer factor antibody, TCF 13 antibody, TCF-13 antibody, TCF13 antibody, TEA domain family member antibody, TEA domain family member 1 (SV40 transcriptional enhancer factor) antibody, TEA domain family member 1 antibody, TEAD 1 antibody, TEAD 1 protein antibody, TEAD-1 antibody, TEAD1 antibody, TEAD1 protein antibody, TEAD1_HUMAN antibody, TEF 1 antibody, TEF1 antibody, Transcription factor 13 (SV40 transcriptional enhancer factor) antibody, Transcription factor 13 antibody, Transcriptional enhancer factor 1 antibody, Transcriptional enhancer factor TEF-1 antibody, Transcriptional enhancer factor TEF1 antibody |
| BACKGROUND |
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| APPLICATION DILUTION |
WB(1:300-1000), IHC-P(1:200-400), IF(ICC)(1:50-200), IHC() |