SUPT5H Monoclonal Antibody
- Size: 100ul
- Application: WB, IHC-P, IF(ICC)
- Reactivity: Human, Mouse, Rat
- Predicted Reactivity:
-
Datasheet
Tech Support
IMAGES
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SUPT5H Monoclonal Antibody, Unconjugated (bsm-54755R) at 1:100 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human thyroid; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SUPT5H Monoclonal Antibody, Unconjugated (bsm-54755R) at 1:100 for 30 minutes at room temperature, DAB staining.
MCF-7 cell;4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SUPT5H) monoclonal Antibody, Unconjugated (bsm-54755R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SUPT5H Monoclonal Antibody, Unconjugated (bsm-54755R) at 1:100 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SUPT5H Monoclonal Antibody, Unconjugated (bsm-54755R) at 1:100 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SUPT5H Monoclonal Antibody, Unconjugated (bsm-54755R) at 1:100 for 30 minutes at room temperature, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
|
|---|---|
|
PRODUCT NAME |
SUPT5H Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic peptide within C-terminal Human SPT5. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
|
|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Nucleus |
| SYNONYMS |
DRB sensitivity inducing factor large subunit antibody, DRB sensitivity-inducing factor 160 kDa subunit antibody, DRB sensitivity-inducing factor large subunit antibody, DSIF large subunit antibody, DSIF p160 antibody, FLJ34157 antibody, hSPT 5 antibody, hSPT5 antibody, SPT 5 antibody, SPT 5H antibody, SPT5 antibody, SPT5H antibody, SPT5H_HUMAN antibody, Suppressor of Ty 5 homolog antibody, Suppressor of Ty 5 homolog (S. cerevisiae) antibody, SUPT 5H antibody, supt5h antibody, Tat cotransactivator 1 protein antibody, Tat CT1 antibody, Tat CT1 protein antibody, Tat-cotransactivator 1 protein antibody, Tat-CT1 protein antibody, Transcription elongation factor SPT 5 antibody, Transcription elongation factor spt5 antibody, Transcription factor Tat CT1 antibody |
| BACKGROUND |
|
| APPLICATION DILUTION |
WB(1:300-1000), IHC-P(1:200-400), IF(ICC)(1:50-200) |