RSK1 p90 Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM, IHC-P, IF(ICC), IHC
- Reactivity: Human, Mouse, Rat
- Predicted Reactivity:
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Datasheet
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IMAGES
Paraformaldehyde-fixed, paraffin embedded Human prostate cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RSK1 p90 Monoclonal Antibody, Unconjugated (bsm-54668R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RSK1 p90 Monoclonal Antibody, Unconjugated (bsm-54668R) at 1:200 for 30 minutes at room temperature, DAB staining.
Flow cytometric analysis of RSK1 p90 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54668R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RSK1 p90 Monoclonal Antibody, Unconjugated (bsm-54668R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RSK1 p90 Monoclonal Antibody, Unconjugated (bsm-54668R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RSK1 p90 Monoclonal Antibody, Unconjugated (bsm-54668R) at 1:200 for 30 minutes at room temperature, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
RSK1 p90 Monoclonal Antibody |
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CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Recombinant protein within N-terminal human RSK1 p90. |
| IMMUNOGEN RANGE |
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| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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| GENE ID |
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| SUBCELLULAR LOCATION |
Cytoplasm, Nucleus |
| SYNONYMS |
90 kDa ribosomal protein S6 kinase 1 antibody, dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody, dJ590P13.1 antibody, EC 2.7.11.1 antibody, HU 1 antibody, HU1 antibody, KS6A1_HUMAN antibody, MAP kinase activated protein kinase 1a antibody, MAP kinase-activated protein kinase 1a antibody, MAPK-activated protein kinase 1a antibody, MAPKAP kinase 1a antibody, MAPKAPK-1a antibody, MAPKAPK1A antibody, MGC79981 antibody, Mitogen-activated protein kinase-activated protein kinase 1A antibody, OTTHUMP00000004113 antibody, p90 RSK1 antibody, p90-RSK 1 antibody, p90rsk antibody, p90RSK1 antibody, p90S6K antibody, pp90RSK1 antibody, Ribosomal protein S6 kinase 90kD 1 antibody, Ribosomal protein S6 kinase 90kD polypeptide 1 antibody, Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody, Ribosomal protein S6 kinase alpha 1 antibody, Ribosomal protein S6 kinase alpha-1 antibody, Ribosomal protein S6 kinase polypeptide 1 antibody, Ribosomal S6 kinase 1 antibody, RPS6K1 alpha antibody, rps6ka antibody, Rps6ka1 antibody, RSK 1 antibody, RSK 1 p90 antibody, RSK antibody, RSK-1 antibody, RSK1 antibody, S6K alpha 1 antibody, S6K-alpha-1 antibody |
| BACKGROUND |
Rsk1 is a member of a family of 90kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine/threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c Fos and CREB. |
| APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100), IHC-P(1:200-400), IF(ICC)(1:50-200), IHC() |