RhoGAP Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM
- Reactivity: Human
- Predicted Reactivity:
-
Datasheet
Tech Support
IMAGES
Siha cells were fixed,permeabilized and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RhoGAP Monoclonal Antibody(bsm-54725R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (red).
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
RhoGAP Monoclonal Antibody |
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CONJUGATION |
Unconjugated |
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HOST |
Rabbit |
| SOURCE |
Synthetic Peptide within C-terminal Human RhoGAP. |
| IMMUNOGEN RANGE |
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| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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| GENE ID |
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| SUBCELLULAR LOCATION |
Cytoplasm, Cell membrane |
| SYNONYMS |
ARHGAP 5 antibody, ARHGAP5 antibody, p190 B antibody, p190 RhoGAP antibody, Rho GTPase activating protein 5 antibody, RhoGAP 5 antibody, RhoGAP antibody, RhoGAP5 antibody |
| BACKGROUND |
Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100) |