p107(Ser975) Polyclonal Antibody
- Size: 100ul
- Application: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
- Reactivity: Human, Mouse, Rat
- Predicted Reactivity: Dog, Cow, Sheep, Pig, Horse
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Datasheet
Tech Support
IMAGES
Lane 1: Rat Testis cell lysates probed with p107(Ser975) Polyclonal Antibody, Unconjugated (bs-5696R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
Human K562 cells probed with p107(Ser975) Polyclonal Antibody, unconjugated (bs-5696R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
p107(Ser975) Polyclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
KLH conjugated synthetic phosphopeptide derived from human RBL1 around the phosphorylation site of Ser975 |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Polyclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Nucleus |
| SYNONYMS |
PRB1; p107; CP107; Retinoblastoma-like protein 1; 107 kDa retinoblastoma-associated protein; RBL1 |
| BACKGROUND |
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor. |
| APPLICATION DILUTION |
WB(1:300-1000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200) |
