NFYA Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM, IHC-P
- Reactivity: Human, Mouse, Rat
- Predicted Reactivity:
-
Datasheet
Tech Support
IMAGES
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFYA Monoclonal Antibody, Unconjugated (bsm-54741R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFYA Monoclonal Antibody, Unconjugated (bsm-54741R) at 1:500 for 30 minutes at room temperature, DAB staining.
THP-1 cells were fixed,permeabilized and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NFYA Monoclonal Antibody(bsm-54741R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (red).
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFYA Monoclonal Antibody, Unconjugated (bsm-54741R) at 1:100 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFYA Monoclonal Antibody, Unconjugated (bsm-54741R) at 1:500 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFYA Monoclonal Antibody, Unconjugated (bsm-54741R) at 1:100 for 30 minutes at room temperature, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
|
|---|---|
|
PRODUCT NAME |
NFYA Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic peptide within C terminal human NFYA. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
|
|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Nucleus |
| SYNONYMS |
CAAT box DNA binding protein subunit A antibody, CAAT box DNA-binding protein subunit A antibody, CBF A antibody, CBF B antibody, CBFA antibody, CBFB antibody, CCAAT binding transcription factor subunit B antibody, FLJ11236 antibody, HAP 2 antibody, HAP2 antibody, HAP2 CCAAT binding protein antibody, NF YA antibody, NF-YA antibody, NFY protein chain A antibody, NFYA antibody, NFYA_HUMAN antibody, NUCLEAR FACTOR BINDING TO Y BOX OF HLA GENES antibody, Nuclear transcription factor Y alpha antibody, Nuclear transcription factor Y antibody, Nuclear transcription factor Y subunit A antibody, Nuclear transcription factor Y subunit alpha antibody, Sez10 antibody, Transcription factor NF Y A subunit antibody |
| BACKGROUND |
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008] |
| APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100), IHC-P(1:200-400) |