LDHD Monoclonal Antibody
- Size: 100ul
- Application: WB, IHC-P, IF(ICC)
- Reactivity: Human, Mouse, Rat
- Predicted Reactivity:
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Datasheet
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IMAGES
Paraformaldehyde-fixed, paraffin embedded Human colon colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LDHD Monoclonal Antibody, Unconjugated (bsm-54704R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LDHD Monoclonal Antibody, Unconjugated (bsm-54704R) at 1:200 for 30 minutes at room temperature, DAB staining.
Siha cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (LDHD) Monoclonal Antibody, Unconjugated (bsm-54704R) 1:50, 90 minutes at 37°C;followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LDHD Monoclonal Antibody, Unconjugated (bsm-54704R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LDHD Monoclonal Antibody, Unconjugated (bsm-54704R) at 1:200 for 30 minutes at room temperature, DAB staining.
HT-29 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (LDHD) Monoclonal Antibody, Unconjugated (bsm-54704R) 1:100, 90 minutes at 37°C;followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
|
PRODUCT NAME |
LDHD Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic Peptide within N-terminal human LDHD. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Mitochondrion |
| SYNONYMS |
D lactate dehydrogenase antibody, DLD antibody, Lactate dehydrogenase D antibody, Ldhd antibody, LDHD_HUMAN antibody, MGC57726 antibody, mitochondrial antibody, Probable D lactate dehydrogenase mitochondrial antibody, Probable D-lactate dehydrogenase antibody |
| BACKGROUND |
LDHD belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. D-lactate and D-glycerate dehydrogenase activities have been found in a similar protein in yeast. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. |
| APPLICATION DILUTION |
WB(1:300-1000), IHC-P(1:200-400), IF(ICC)(1:50-200) |