PRODUCT NAME

LATS1 (Thr1079) Polyclonal Antibody, Cy3 Conjugated

CONJUGATION

Cy3

HOST

Rabbit

SOURCE

KLH conjugated synthetic phosphopeptide derived from human LATS1 around the phosphorylation site of Thr1079

IMMUNOGEN RANGE

CLONALITY

Polyclonal

ISOTYPE

IgG

CONCENTRATION

1ug/ul

PURIFICATION

Purified by Protein A.

STORAGE BUFFER

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

STORAGE CONDITION

Store at 4°C for 12 months.

GENE ID

9113

SUBCELLULAR LOCATION

Cytoplasm

SYNONYMS

wts; WARTS; Serine/threonine-protein kinase LATS1; Large tumor suppressor homolog 1; WARTS protein kinase; h-warts; LATS1

BACKGROUND

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.

APPLICATION DILUTION

IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)