IGF 1 Polyclonal Antibody
- Size: 100ul
- Application: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP
- Reactivity: Human, Mouse, Rat, Dog
- Predicted Reactivity: Cow, Sheep, Pig, Rabbit
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Datasheet
Tech Support
IMAGES
Mouse liver lysates probed with Rabbit Anti-IGF-1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IGF 1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:200 overnight at 4°C, DAB staining.
Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
PRODUCT DETAILS
SPECIFICATIONS |
|
|---|---|
|
PRODUCT NAME |
IGF 1 Polyclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
KLH conjugated synthetic peptide derived from human IGF-1 |
| IMMUNOGEN RANGE |
49-100/195 |
| CLONALITY |
Polyclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
|
|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Secreted |
| SYNONYMS |
IGFI; IGF-I; IGF1A; Insulin-like growth factor I; Mechano growth factor; MGF; Somatomedin-C; IGF1; IBP1 |
| BACKGROUND |
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. |
| APPLICATION DILUTION |
WB(1:300-1000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200), IP(1-2ug) |
