Human Kappa light chain
- Size: 100ul
- Application: WB, IHC-P
- Reactivity: Human
- Predicted Reactivity:
-
Datasheet
Tech Support
IMAGES
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Human Kappa light chain Monoclonal Antibody, Unconjugated (bsm-54763R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Human Kappa light chain Monoclonal Antibody, Unconjugated (bsm-54763R) at 1:200 overnight at 4°C, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
|
|---|---|
|
PRODUCT NAME |
Human Kappa light chain |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Full length native human IgG. |
| IMMUNOGEN RANGE |
1-107/107 |
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Extracellular, Secreted |
| SYNONYMS |
Kappa Light Chain; IGKC; HCAK 1; HCAK1; Ig kappa chain C region; IGKC; Immunoglobulin kappa constant; Immunoglobulin kappa constant region; Immunoglobulin kappa light chain; Kappa 1 immunoglobulin light chain; Km; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809; Human Ig/L-Chain Kappa; IGKC_HUMAN. |
| BACKGROUND |
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. |
| APPLICATION DILUTION |
WB(1:300-1000), IHC-P(1:200-400) |