GSK-3 beta (NT) Polyclonal Antibody
- Size: 100ul
- Application: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
- Reactivity: Human, Mouse, Zebrafish
- Predicted Reactivity: Rat, Dog, Cow, Sheep, Pig, Horse, Chicken
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Datasheet
Tech Support
IMAGES
Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GSK-3 beta) polyclonal Antibody, Unconjugated (bs-0023M) 1:100, 90 minutes at 37°C; followed by a CY5 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (CD44v3) polyclonal Antibody, Unconjugated (bs-4497R) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Mouse brain lysates probed with Mouse Anti-GSK-3 beta Polyclonal Antibody, Unconjugated (bs-0023M) at 1:200 overnight at 4°C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GSK-3 beta (NT) Polyclonal Antibody, Unconjugated (bs-0023M) at 1:200 overnight at 4°C, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
GSK-3 beta (NT) Polyclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Mouse |
| SOURCE |
KLH conjugated synthetic peptide derived from human GSK-3 Beta |
| IMMUNOGEN RANGE |
1-50/420 |
| CLONALITY |
Polyclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Cytoplasm, Nucleus, Cell membrane |
| SYNONYMS |
Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B |
| BACKGROUND |
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
| APPLICATION DILUTION |
WB(1:300-1000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200), ICC(1:100-500) |