ERK1 + ERK2 Polyclonal Antibody
- Size: 100ul
- Application: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
- Reactivity: Human, Mouse, Rat, Pig
- Predicted Reactivity: Dog, Cow, Horse, Chicken, Rabbit
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Datasheet
Tech Support
IMAGES
L1 rat brain, L2 rat heart lysates probed (bs-0022R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band and observed band size: 42kDa.
Formalin-fixed and paraffin embedded rat hippocampus labeled with Anti-ERK2/MAPK1 Polyclonal Antibody, Unconjugated (bs-0022R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Mouse Lymph node lysates; Lane 4: Rat Lymph node cell lysates probed with ERK1 + ERK2 Polyclonal Antibody, Unconjugated (bs-0022R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-ERK2/MAPK1 Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C.
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-ERK2/MAPK1 Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-ERK2/MAPK1 Polyclonal Antibody, Unconjugated (bs-0022R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
ERK1 + ERK2 Polyclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
KLH conjugated synthetic peptide derived from mouse ERK2 |
| IMMUNOGEN RANGE |
301-358/358 |
| CLONALITY |
Polyclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Cytoplasm, Nucleus, Cell membrane, Extracellular matrix |
| SYNONYMS |
Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; Mapk1; Erk2; Mapk; Prkm1 |
| BACKGROUND |
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. |
| APPLICATION DILUTION |
WB(1:300-1000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200) |
