Elongation factor 1-gamma Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM, IHC-P, IF(ICC)
- Reactivity: Human, Mouse, Rat
- Predicted Reactivity:
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Datasheet
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IMAGES
Paraformaldehyde-fixed, paraffin embedded Human intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:400 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:400 for 30 minutes at room temperature, DAB staining.
Daudi cells were fixed with 4% PFA for 10min at room temperature,permeabilized with for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Elongation factor 1-gamma Monoclonal Antibody(bsm-54719R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green).
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:400 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Elongation factor 1-gamma Monoclonal Antibody, Unconjugated (bsm-54719R) at 1:400 for 30 minutes at room temperature, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Elongation factor 1-gamma Monoclonal Antibody |
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CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic Peptide within N terminal human Elongation factor 1-gamma. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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| GENE ID |
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| SUBCELLULAR LOCATION |
Cytoplasm, Nucleus, Extracellular exosome, Membrane |
| SYNONYMS |
2610301D06Rik antibody, AA407312 antibody, eEF 1B gamma antibody, EEF 1G antibody, eEF-1B gamma antibody, EEF1G antibody, EF 1 gamma antibody, EF 1G antibody, EF-1-gamma antibody, EF1 gamma antibody, EF1G antibody, EF1G_HUMAN antibody, Elongation factor 1 gamma antibody, Elongation factor 1-gamma antibody, Eukaryotic translation elongation factor 1 gamma antibody, GIG 35 antibody, GIG35 antibody, MGC103354 antibody, MGC114210 antibody, MGC144723 antibody, MGC144724 antibody, MGC94929 antibody, Pancreatic tumor related protein antibody, PRO1608 antibody, Translation elongation factor eEF 1 gamma chain antibody |
| BACKGROUND |
EF-1 (elongation factor-1) is a multi-protein complex that is responsible for the delivery of aminoacyl-tRNAs to the ribosome. EF-1 gamma (elongation factor 1-gamma), also known as EEF1G or GIG35, is a 437 amino acid subunit of the EF-1 complex. Expressed in stomach, pancreas, brain, lung, kidney, intestine, liver and spleen, EF-1 gamma contains an N-terminal glutathione transferase domain which is thought to be involved in anchoring the complex to various cellular components. Additionally, EF-1 gamma may play a key role in the assembly of multiprotein complexes containing aminoacyl-tRNA synthetases. Increased expression of EF-1 gamma is associated with pancreatic cancer, suggesting a possible role for EF-1 gamma in the oncogenic transformation process. |
| APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100), IHC-P(1:200-400), IF(ICC)(1:50-200) |