eIF3e Monoclonal Antibody
- Size: 100ul
- Application: WB, IHC-P
- Reactivity: Human, Mouse, Rat
- Predicted Reactivity:
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Datasheet
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IMAGES
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with eIF3e Monoclonal Antibody, Unconjugated (bsm-54714R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with eIF3e Monoclonal Antibody, Unconjugated (bsm-54714R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with eIF3e Monoclonal Antibody, Unconjugated (bsm-54714R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with eIF3e Monoclonal Antibody, Unconjugated (bsm-54714R) at 1:50 for 30 minutes at room temperature, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
|
PRODUCT NAME |
eIF3e Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic Peptide corresponding to N terminal human eIF3e. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Cytoplasm, PML body |
| SYNONYMS |
eIF-3 p48 antibody, eIF3e antibody, EIF3E_HUMAN antibody, EIF3S6 antibody, eIFe antibody, Eukaryotic translation initiation factor 3 subunit 6 antibody, Eukaryotic translation initiation factor 3 subunit E antibody, eukaryotic translation initiation factor 3, subunit 6 (48kD) antibody, INT6 antibody, mammary tumor-associated protein INT6 antibody, murine mammary tumor integration site 6 (oncogene homolog) antibody, Viral integration site protein INT-6 homolog antibody |
| BACKGROUND |
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| APPLICATION DILUTION |
WB(1:300-1000), IHC-P(1:200-400) |