ECM1 Monoclonal Antibody
- Size: 100ul
- Application: WB, IHC-P, IF(ICC)
- Reactivity: Human, Rat
- Predicted Reactivity:
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Datasheet
Tech Support
IMAGES
SiHa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ECM1) monoclonal Antibody, Unconjugated (bsm-54715R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ECM1 Monoclonal Antibody, Unconjugated (bsm-54715R) at 1:50 for 30 minutes at room temperature, DAB staining.
MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ECM1) monoclonal Antibody, Unconjugated (bsm-54715R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
ECM1 Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic Peptide within N terminal human ECM1. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
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| SUBCELLULAR LOCATION |
Extracellular matrix |
| SYNONYMS |
ECM 1 antibody, Ecm1 antibody, ECM1_HUMAN antibody, Extracellular matrix protein 1 antibody, Secretory component p85 antibody, URBWD antibody |
| BACKGROUND |
Extracellular matrix protein 1 (ECM1) This family consists of several eukaryotic extracellular matrix protein 1 (ECM1) sequences. ECM1 has been shown to regulate endochondral bone formation, stimulate the proliferation of endothelial cells and induce angiogenesis. Mutations in the ECM1 gene can cause lipoid proteinosis, a disorder which causes generalised thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. |
| APPLICATION DILUTION |
WB(1:300-1000), IHC-P(1:200-400), IF(ICC)(1:50-200) |