DBF4 Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM
- Reactivity: Human, Mouse
- Predicted Reactivity:
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Datasheet
Tech Support
IMAGES
Flow cytometric analysis of DBF4 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54705R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
DBF4 Monoclonal Antibody |
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CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic Peptide within N-terminal human DBF4. |
| IMMUNOGEN RANGE |
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| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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| GENE ID |
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| SUBCELLULAR LOCATION |
Nucleus |
| SYNONYMS |
Activator of S phase kinase antibody, ASK antibody, CHIF antibody, Chiffon homolog A antibody, DBF4 antibody, DBF4 homolog (S. cerevisiae) antibody, DBF4 homolog antibody, DBF4 type zinc finger containing protein 1 antibody, DBF4 zinc finger A antibody, DBF4 zinc finger antibody, DBF4, S. cerevisiae, homolog of antibody, DBF4-type zinc finger-containing protein 1 antibody, DBF4A antibody, DBF4A_HUMAN antibody, Dumbbell former 4, S. cerevisiae, homolog of antibody, OTTHUMP00000161661 antibody, Protein DBF4 homolog A antibody, ZDBF1 antibody, Zinc finger, DBF-type containing 1 antibody |
| BACKGROUND |
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle. Tissue specificity: Highly expressed in testis and thymus. Expressed also in most cancer cells lines. |
| APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100) |